This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ARHGAP23, Human
PrimePCR™ Template for Probe Assay: ARHGAP23, Human
The RHO (see ARHA; MIM 165390) family of small GTPases are involved in signal transduction through transmembrane receptors and they are inactive in the GDP-bound form and active in the GTP-bound form. GTPase-activating proteins such as ARHGAP23 inactivate RHO family proteins by stimulating their hydrolysis of GTP (Katoh and Katoh 2004 [PubMed 15254754]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.