PrimePCR™ Probe Assay: CELA3B, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041110
Assay Design:   Intron-spanning
Chromosome Location:   1:22307531-22310246question
Amplicon Length:   165
Splice Variants Targeted:   ENST00000598205 ENST00000601313 ENST00000596571 ENST00000440075 ENST00000376970 ENST00000425460 ENST00000262269 ENST00000252575 ENST00000538881 ENST00000332145 ENST00000418929 ENST00000420890 ENST00000489426 ENST00000308354 ENST00000317613 ENST00000374136 ENST00000379558 ENST00000403861 ENST00000374666 ENST00000337107 ENST00000595821

Gene Information

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1 2 2A 2B 3A and 3B. Unlike other elastases elastase 3B has little elastolytic activity. Like most of the human elastases elastase 3B is secreted from the pancreas as a zymogen and like other serine proteases such as trypsin chymotrypsin and kallikrein it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1 and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. [provided by RefSeq May 2009]

Gene Symbol:   CELA3B
Gene Name:   chymotrypsin-like elastase family, member 3B
Aliases:   CBPP, E1, EL-1, ELA3B
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000219073
Entrez:   23436
UniGene:   Hs.728752
Chromosome Mapping:   1p36.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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