PrimePCR™ Probe Assay: HIBCH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HIBCH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIBCH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041022
Assay Design:   Intron-spanning
Chromosome Location:   2:191077724-191114372question
Amplicon Length:   195
Splice Variants Targeted:   ENST00000261275 ENST00000378723 ENST00000358062 ENST00000378677 ENST00000357033 ENST00000378702 ENST00000465285 ENST00000474231 ENST00000361471 ENST00000378680 ENST00000359836 ENST00000343523 ENST00000378707 ENST00000541735 ENST00000397707 ENST00000560611 ENST00000354181 ENST00000458406 ENST00000397702 ENST00000560164 ENST00000290209 ENST00000558667 ENST00000558589 ENST00000451844 ENST00000300176 ENST00000392332 ENST00000359678 ENST00000410045 ENST00000416732 ENST00000409820 ENST00000576518

Gene Information

This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   HIBCH
Gene Name:   3-hydroxyisobutyryl-CoA hydrolase
Aliases:   HIBYLCOAH
RefSeq:   NC_000002.11 NG_017062.1 NT_005403.17
Ensembl:   ENSG00000198130
Entrez:   26275
Chromosome Mapping:   2q32.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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