PrimePCR™ Probe Assay: AKR1CL1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040991
Assay Design:   Intron-spanning
Chromosome Location:   10:5204879-5227094question
Amplicon Length:   195
Splice Variants Targeted:   ENST00000445041 ENST00000555262 ENST00000449574 ENST00000308868 ENST00000430737 ENST00000422962 ENST00000547669 ENST00000549768 ENST00000278064 ENST00000357060 ENST00000428666 ENST00000458561 ENST00000414956 ENST00000547056 ENST00000549383 ENST00000376007 ENST00000376033 ENST00000454306 ENST00000432252 ENST00000550609 ENST00000550109 ENST00000439762 ENST00000416335 ENST00000550674 ENST00000548373 ENST00000401000 ENST00000369068 ENST00000596341 ENST00000435971 ENST00000428775 ENST00000551794 ENST

Gene Information

Description Not Available

Gene Symbol:   AKR1CL1
Gene Name:   aldo-keto reductase family 1, member C-like 1
Aliases:   FLJ16347
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000196326
Entrez:   340811
UniGene:   Hs.282513
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.620000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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