PrimePCR™ Probe Assay: NXF2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040961
Assay Design:   Intron-spanning
Chromosome Location:   X:101503513-101571929question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000547525 ENST00000332082 ENST00000549903 ENST00000261400 ENST00000361971 ENST00000538966 ENST00000268719 ENST00000368808 ENST00000341689 ENST00000567679 ENST00000371692 ENST00000371691 ENST00000372763 ENST00000372758 ENST00000372757 ENST00000395088 ENST00000330252 ENST00000340622 ENST00000458143 ENST00000372752 ENST00000372749 ENST00000372750 ENST00000457521 ENST00000412230 ENST00000354333 ENST00000350796 ENST00000303391 ENST00000272091 ENST00000336576 ENST00000243776 ENST00000355327 ENST

Gene Information

This gene is one of a family of nuclear RNA export factor genes. It encodes a protein that is involved in mRNA export is located in the nucleoplasm and is associated with the nuclear envelope. Alternative splicing seems to be a common mechanism in this gene family. Two variants have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NXF2
Gene Name:   nuclear RNA export factor 2
Aliases:   CT39, FLJ20416, NXF2B, TAPL-2
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000185554
Entrez:   56001
UniGene:   Hs.530358
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.870000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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