PrimePCR™ Probe Assay: NPIP, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040949
Assay Design:   Intron-spanning
Chromosome Location:   16:15039794-15042891question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000399147 ENST00000547494 ENST00000526520 ENST00000531598 ENST00000552140 ENST00000530328 ENST00000529166 ENST00000553201 ENST00000328085 ENST00000424549 ENST00000537062 ENST00000534799 ENST00000547107 ENST00000358815 ENST00000360151 ENST00000534094 ENST00000543801 ENST00000432570 ENST00000381497 ENST00000524823 ENST00000527703 ENST00000331436 ENST00000541593 ENST00000530217 ENST00000422673 ENST00000327792 ENST00000525596 ENST00000545050 ENST00000545114 ENST00000427999 ENST00000528301 ENST

Gene Information

Description Not Available

Gene Symbol:   NPIP
Gene Name:   nuclear pore complex interacting protein
Aliases:   FLJ42525, FLJ44848
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000183426
Entrez:   9284
UniGene:   Hs.676266
Chromosome Mapping:   16p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.420000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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