PrimePCR™ Probe Assay: FAM153B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAM153B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAM153B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040939
Assay Design:   Intron-spanning
Chromosome Location:   5:175526024-175530253question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000412319 ENST00000316310 ENST00000510276 ENST00000440605 ENST00000295890 ENST00000507544 ENST00000343508 ENST00000297405 ENST00000455883 ENST00000510151 ENST00000515817 ENST00000503724 ENST00000253490 ENST00000475164 ENST00000273580 ENST00000473092 ENST00000347147 ENST00000381975 ENST00000392432 ENST00000593751 ENST00000596733

Gene Information

Description Not Available

Gene Symbol:   FAM153B
Gene Name:   family with sequence similarity 153, member B
Aliases:   DKFZp434D115
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000182230
Entrez:   202134
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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