PrimePCR™ Probe Assay: GTF2IRD2B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040904
Assay Design:   Intron-spanning
Chromosome Location:   7:74554805-74558323question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000324896 ENST00000353920 ENST00000346152 ENST00000416070 ENST00000405086 ENST00000361071 ENST00000453619 ENST00000356115 ENST00000312575 ENST00000394939 ENST00000430511 ENST00000573035

Gene Information

This gene encodes a glycosylated phosphoprotein with a leucine zipper motif two helix-loop-helix motifs (I repeats) that are similar to domains found in the TFII-I family of transcription factors one CHARLIE8 transposable element-like sequence and a BED zinc finger. This gene lies within a region that is deleted in Williams-Beuren syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however not all variants have been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   GTF2IRD2B
Gene Name:   GTF2I repeat domain containing 2B
Aliases:   Not Available
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000174428
Entrez:   389524
UniGene:   Not Available
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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