PrimePCR™ Probe Assay: ADCK5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ADCK5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADCK5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040899
Assay Design:   Intron-spanning
Chromosome Location:   8:145603076-145608406question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000564001 ENST00000569475 ENST00000563699 ENST00000589261 ENST00000296452 ENST00000368078 ENST00000368079 ENST00000301904 ENST00000254695 ENST00000366401 ENST00000308860 ENST00000532190 ENST00000296776 ENST00000426079 ENST00000334126 ENST00000449707 ENST00000411696 ENST00000277165 ENST00000427765 ENST00000333936 ENST00000340893 ENST00000215957

Gene Information

Description Not Available

Gene Symbol:   ADCK5
Gene Name:   aarF domain containing kinase 5
Aliases:   FLJ35454, MGC126708
RefSeq:   NC_000008.10 NT_037704.5 NW_003315924.1
Ensembl:   ENSG00000173137
Entrez:   203054
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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