PrimePCR™ Probe Assay: C9orf7, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040825
Assay Design:   Intron-spanning
Chromosome Location:   9:136325241-136328650question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000378628 ENST00000466533 ENST00000484460 ENST00000449135 ENST00000542554 ENST00000543642 ENST00000264712 ENST00000405914 ENST00000291722 ENST00000316948 ENST00000540581 ENST00000542192 ENST00000370899 ENST00000557284 ENST00000326637 ENST00000370891 ENST00000307741 ENST00000586677 ENST00000591363 ENST00000590970 ENST00000587401 ENST00000541714 ENST00000430249 ENST00000233072 ENST00000451903 ENST00000570003 ENST00000568974 ENST00000593312 ENST00000599066 ENST00000593805 ENST00000600043 ENST

Gene Information

Description Not Available

Gene Symbol:   C9orf7
Gene Name:   chromosome 9 open reading frame 7
Aliases:   D9S2135, FLOWER
RefSeq:   NG_011934.1 NC_000009.11 NT_035014.4 NW_003315925.1
Ensembl:   ENSG00000160325
Entrez:   11094
UniGene:   Hs.62003
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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