PrimePCR™ Probe Assay: SAE1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040788
Assay Design:   Intron-spanning
Chromosome Location:   19:47653516-47656180question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000280333 ENST00000594144 ENST00000594526 ENST00000597808 ENST00000413379 ENST00000600706 ENST00000600753 ENST00000270225 ENST00000392776 ENST00000403299 ENST00000406271 ENST00000251973

Gene Information

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912) or sumoylation regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al. 1999 [PubMed 9920803]).[supplied by OMIM Mar 2010]

Gene Symbol:   SAE1
Gene Name:   SUMO1 activating enzyme subunit 1
Aliases:   AOS1, FLJ3091, HSPC140, SUA1, UBLE1A
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142230
Entrez:   10055
UniGene:   Hs.515500
Chromosome Mapping:   19q13.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.800000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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