PrimePCR™ Probe Assay: PRRG1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040752
Assay Design:   Intron-spanning
Chromosome Location:   X:37265462-37285218question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000378628 ENST00000466533 ENST00000484460 ENST00000449135 ENST00000463135 ENST00000542554 ENST00000543642 ENST00000465127 ENST00000338983 ENST00000338641 ENST00000361452 ENST00000413209 ENST00000347330 ENST00000593312 ENST00000599066 ENST00000593805 ENST00000600043 ENST00000600993 ENST00000597842 ENST00000594336 ENST00000596599

Gene Information

This gene encodes a vitamin K-dependent gamma-carboxyglutamic acid (Gla)-containing single-pass transmembrane protein. This protein contains a Gla domain at the N-terminus preceded by a propeptide sequence required for post-translational gamma-carboxylation of specific glutamic acid residues by a vitamin K-dependent gamma-carboxylase. The C-terminus is proline-rich containing PPXY and PXXP motifs found in a variety of signaling and cytoskeletal proteins. This gene is highly expressed in the spinal cord. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   PRRG1
Gene Name:   proline rich Gla (G-carboxyglutamic acid) 1
Aliases:   PRGP1
RefSeq:   NC_000023.10 NG_016770.1 NT_079573.4
Ensembl:   ENSG00000130962
Entrez:   5638
UniGene:   Hs.190341
Chromosome Mapping:   Xp21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.670000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download