PrimePCR™ Probe Assay: CNTNAP3, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040699
Assay Design:   Intron-spanning
Chromosome Location:   9:39149946-39171420question
Amplicon Length:   198
Splice Variants Targeted:   ENST00000286614 ENST00000372129 ENST00000301740 ENST00000297668 ENST00000358144 ENST00000377659 ENST00000377656 ENST00000369833 ENST00000377564 ENST00000302805 ENST00000372409 ENST00000393405 ENST00000359238 ENST00000256646 ENST00000347785 ENST00000343905 ENST00000307194 ENST00000397560 ENST00000581899

Gene Information

The protein encoded by this gene belongs to the NCP family of cell-recognition molecules. This family represents a distinct subgroup of the neurexins. NCP proteins mediate neuron-glial interactions in vertebrates and glial-glial contact in invertebrates. The protein encoded by this gene may play a role in cell recognition within the nervous system. Alternatively spliced transcript variants encoding different isoforms have been described but their biological nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CNTNAP3
Gene Name:   contactin associated protein-like 3
Aliases:   CASPR3, CNTNAP3A, RP11-138L21.1, RP11-290L7.1
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000106714
Entrez:   79937
UniGene:   Hs.128474
Chromosome Mapping:   9p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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