PrimePCR™ Probe Assay: TSGA14, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040697
Assay Design:   Intron-spanning
Chromosome Location:   7:130044483-130052037question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000264042 ENST00000223208 ENST00000343969 ENST00000492389 ENST00000472739 ENST00000477003 ENST00000475282 ENST00000541543 ENST00000264036 ENST00000392814 ENST00000368773 ENST00000580443 ENST00000583790 ENST00000577507 ENST00000578060 ENST00000579704 ENST00000582998 ENST00000582105

Gene Information

Description Not Available

Gene Symbol:   TSGA14
Gene Name:   testis specific, 14
Aliases:   Cep41, DKFZp762H1311
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106477
Entrez:   95681
UniGene:   Not Available
Chromosome Mapping:   7q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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