PrimePCR™ Probe Assay: PQBP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PQBP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PQBP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040683
Assay Design:   Intron-spanning
Chromosome Location:   X:48755821-48758537question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000447146 ENST00000247140 ENST00000443648 ENST00000376563 ENST00000376566 ENST00000218224 ENST00000396763 ENST00000376548 ENST00000597699 ENST00000600640 ENST00000600107 ENST00000597809 ENST00000601234 ENST00000594868 ENST00000593464 ENST00000602235

Gene Information

This gene encodes a nuclear polyglutamine-binding protein that is involved with transcription activation. The encoded protein contains a WW domain. Mutations in this gene have been found in patients with Renpenning syndrome 1 and other syndromes with X-linked mental retardation. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.[provided by RefSeq Nov 2009]

Gene Symbol:   PQBP1
Gene Name:   polyglutamine binding protein 1
Aliases:   MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS
RefSeq:   NC_000023.10 NG_015968.1 NT_079573.4 NG_015967.1
Ensembl:   ENSG00000102103
Entrez:   10084
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.550000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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