PrimePCR™ Probe Assay: YTHDC1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040664
Assay Design:   Intron-spanning
Chromosome Location:   4:69182059-69184558question
Amplicon Length:   195
Splice Variants Targeted:   ENST00000550485 ENST00000552105 ENST00000401787 ENST00000381306 ENST00000381309 ENST00000535193 ENST00000543771 ENST00000536108 ENST00000602795 ENST00000344018 ENST00000344157 ENST00000355665 ENST00000579690 ENST00000507529

Gene Information

Description Not Available

Gene Symbol:   YTHDC1
Gene Name:   YTH domain containing 1
Aliases:   KIAA1966, YT521, YT521-B
RefSeq:   NC_000004.11 NT_022778.16 NT_167250.1
Ensembl:   ENSG00000083896
Entrez:   91746
UniGene:   Hs.175955
Chromosome Mapping:   4q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.980000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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