PrimePCR™ Probe Assay: PKM2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040657
Assay Design:   Intron-spanning
Chromosome Location:   15:72509750-72511317question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000565184 ENST00000335181 ENST00000565154 ENST00000568459 ENST00000561609 ENST00000564178 ENST00000562997 ENST00000567087 ENST00000566809 ENST00000319622 ENST00000389093 ENST00000243077 ENST00000533050 ENST00000360194 ENST00000369144 ENST00000541549 ENST00000205636

Gene Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq May 2011]

Gene Symbol:   PKM2
Gene Name:   pyruvate kinase, muscle
Aliases:   CTHBP, MGC3932, OIP3, PK3, PKM, TCB, THBP1
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000067225
Entrez:   5315
UniGene:   Hs.534770
Chromosome Mapping:   15q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.670000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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