PrimePCR™ Probe Assay: DENND1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DENND1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DENND1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040620
Assay Design:   Intron-spanning
Chromosome Location:   1:197621374-197627498question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000235453 ENST00000367396 ENST00000422998 ENST00000400967

Gene Information

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family such as DENND1B function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson 2010 [PubMed 20154091]).[supplied by OMIM Nov 2010]

Gene Symbol:   DENND1B
Gene Name:   DENN/MADD domain containing 1B
Aliases:   C1ORF18, C1orf218, DKFZp547O0715, FAM31B, FLJ20054, FLJ21179, FLJ33888, MGC103810, MGC27044
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000213047
Entrez:   163486
Chromosome Mapping:   1q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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