This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MDFIC, Human
PrimePCR™ Template for Probe Assay: MDFIC, Human
This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG) and an in-frame downstream AUG codon results in the production of two isoforms p40 and p32 respectively which have different subcellular localization; p32 is mainly found in the cytoplasm whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.