This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: LLGL2, Human
PrimePCR™ Template for Probe Assay: LLGL2, Human
The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division epithelial cell polarity and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.