PrimePCR™ Probe Assay: RP11-231C14.2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RP11-231C14.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-231C14.2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040264
Assay Design:   Intron-spanning
Chromosome Location:   16:29401570-29405357question
Amplicon Length:   159
Splice Variants Targeted:   ENST00000524087 ENST00000415833 ENST00000538606 ENST00000517539 ENST00000528249 ENST00000424340 ENST00000520915 ENST00000550665 ENST00000504841 ENST00000419180 ENST00000521589 ENST00000448012 ENST00000458643 ENST00000341400 ENST00000518761 ENST00000415645 ENST00000357370 ENST00000451409 ENST00000399147 ENST00000547494 ENST00000538896

Gene Information

Description Not Available

Gene Symbol:   RP11-231C14.2
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000254206
Entrez:   728888
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 37.360000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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