PrimePCR™ Probe Assay: RNF103-VPS24, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040252
Assay Design:   Intron-spanning
Chromosome Location:   2:86756453-86847473question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000270642 ENST00000443236 ENST00000278947 ENST00000411734 ENST00000574091 ENST00000574002 ENST00000219593 ENST00000587515 ENST00000336926 ENST00000434438 ENST00000439940 ENST00000440757 ENST00000575057 ENST00000576304

Gene Information

Description Not Available

Gene Symbol:   RNF103-VPS24
Gene Name:   RNF103-VPS24 readthrough
Aliases:   FLJ58988
RefSeq:   Not Available
Ensembl:   ENSG00000249884
Entrez:   100526767
UniGene:   Not Available
Chromosome Mapping:   2p

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.610000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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