PrimePCR™ Probe Assay: NME1-NME2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040232
Assay Design:   Intron-spanning
Chromosome Location:   17:49247352-49248930question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000393193 ENST00000555572 ENST00000393198 ENST00000393185 ENST00000393183 ENST00000514264 ENST00000513177 ENST00000512737 ENST00000503064 ENST00000393190 ENST00000270139 ENST00000299855 ENST00000572537

Gene Information

Description Not Available

Gene Symbol:   NME1-NME2
Gene Name:   NME1-NME2 readthrough
Aliases:   FLJ93990, NM23-LV, NMELV
RefSeq:   NC_000017.10 NG_021169.1 NT_010783.15
Ensembl:   ENSG00000243678
Entrez:   654364
UniGene:   Not Available
Chromosome Mapping:   17q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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