PrimePCR™ Probe Assay: HLA-DQA2, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040206
Assay Design:   Intron-spanning
Chromosome Location:   6:32709259-32712986question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000340780 ENST00000342114 ENST00000371776 ENST00000539850 ENST00000449560 ENST00000546801 ENST00000372034 ENST00000372032 ENST00000453504 ENST00000457246 ENST00000442315 ENST00000450960 ENST00000299980 ENST00000564155 ENST00000569748 ENST00000393512 ENST00000433195 ENST00000423132 ENST00000447735 ENST00000551533 ENST00000456258 ENST00000450614 ENST00000441297 ENST00000420385 ENST00000241802 ENST00000548145 ENST00000550239 ENST00000552745 ENST00000546887 ENST00000443184 ENST00000383251 ENST

Gene Information

This gene belongs to the HLA class II alpha chain family. The encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B lymphocytes dendritic cells macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells. [provided by RefSeq Jun 2010]

Gene Symbol:   HLA-DQA2
Gene Name:   major histocompatibility complex, class II, DQ alpha 2
Aliases:   DX-ALPHA, HLA-DXA
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000237541
Entrez:   3118
UniGene:   Not Available
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.130000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download