PrimePCR™ Probe Assay: MFRP, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040195
Assay Design:   Intron-spanning
Chromosome Location:   11:119213642-119214578question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000445041 ENST00000555262 ENST00000449574 ENST00000395325 ENST00000371069 ENST00000263441

Gene Information

This gene encodes a member of the frizzled-related proteins. The encoded protein may play a role in eye development as mutations in this gene have been associated with nanophthalmos posterior microphthalmia retinitis pigmentosa foveoschisis and optic disc drusen. The protein is encoded by a bicistronic mRNA which also encodes C1q and tumor necrosis factor related protein 5.[provided by RefSeq Feb 2009]

Gene Symbol:   MFRP
Gene Name:   membrane frizzled-related protein
Aliases:   DKFZp586B0621, FLJ30570, MCOP5, MGC32938, NNO2, RD6
RefSeq:   NC_000011.9 NG_009626.1 NG_012235.1 NT_033899.8
Ensembl:   ENSG00000235718
Entrez:   83552
UniGene:   Not Available
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.030000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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