This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MFRP, Human
PrimePCR™ Template for Probe Assay: MFRP, Human
This gene encodes a member of the frizzled-related proteins. The encoded protein may play a role in eye development as mutations in this gene have been associated with nanophthalmos posterior microphthalmia retinitis pigmentosa foveoschisis and optic disc drusen. The protein is encoded by a bicistronic mRNA which also encodes C1q and tumor necrosis factor related protein 5.[provided by RefSeq Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.