PrimePCR™ Probe Assay: PRH1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040179
Assay Design:   Intron-spanning
Chromosome Location:   12:11035224-11036790question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000573223 ENST00000572835 ENST00000428168 ENST00000381847 ENST00000396400 ENST00000358369 ENST00000406926 ENST00000277475 ENST00000320955 ENST00000295190 ENST00000457406 ENST00000412034 ENST00000575657 ENST00000572141

Gene Information

Description Not Available

Gene Symbol:   PRH1
Gene Name:   proline-rich protein HaeIII subfamily 1
Aliases:   MGC74956, PA
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000231887
Entrez:   5554
UniGene:   Hs.656965
Chromosome Mapping:   12p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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