PrimePCR™ Probe Assay: CLIC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CLIC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CLIC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040118
Assay Design:   Intron-spanning
Chromosome Location:   6:31698644-31700052question
Amplicon Length:   166
Splice Variants Targeted:   ENST00000349499 ENST00000360731 ENST00000538167 ENST00000360737 ENST00000309469 ENST00000540862 ENST00000254695 ENST00000366401 ENST00000360010 ENST00000425464 ENST00000423143 ENST00000438750 ENST00000451546 ENST00000478951 ENST00000273368 ENST00000393917 ENST00000456863 ENST00000434202 ENST00000420458 ENST00000433916 ENST00000395824 ENST00000395825 ENST00000375784 ENST00000375779 ENST00000375780 ENST00000395892 ENST00000418285 ENST00000423055 ENST00000442045 ENST00000435242 ENST00000422167 ENST

Gene Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential transepithelial transport maintenance of intracellular pH and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq Jul 2008]

Gene Symbol:   CLIC1
Gene Name:   chloride intracellular channel 1
Aliases:   G6, NCC27
RefSeq:   NC_000006.11 NG_011611.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000213719
Entrez:   1192
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.460000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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