PrimePCR™ Probe Assay: TXNRD3NB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TXNRD3NB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TXNRD3NB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040110
Assay Design:   Intron-spanning
Chromosome Location:   3:126291366-126326602question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000389665 ENST00000356083 ENST00000383572 ENST00000360079 ENST00000402580 ENST00000359308 ENST00000405878 ENST00000405506 ENST00000428575 ENST00000302101 ENST00000370481 ENST00000370473 ENST00000370466 ENST00000370459 ENST00000443807 ENST00000343435 ENST00000245934 ENST00000263228 ENST00000280734 ENST00000368653 ENST00000368654 ENST00000589049 ENST00000367137 ENST00000336715 ENST00000324048 ENST00000426871 ENST00000368879 ENST00000367002 ENST00000324589 ENST00000540857 ENST00000344227 ENST

Gene Information

Description Not Available

Gene Symbol:   TXNRD3NB
Gene Name:   thioredoxin reductase 3 neighbor
Aliases:   MGC163418, MGC163420, TR2IT1, TXNRD3IT1, TXNRD3NT1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000206483
Entrez:   645840
Chromosome Mapping:   3q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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