PrimePCR™ Probe Assay: DAZ4, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040105
Assay Design:   Intron-spanning
Chromosome Location:   Y:27000980-27003460question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000407540 ENST00000421259 ENST00000405239 ENST00000382510 ENST00000426000 ENST00000540248 ENST00000382449 ENST00000382440 ENST00000382433 ENST00000382306 ENST00000449947 ENST00000382294 ENST00000382424 ENST00000400493 ENST00000382431 ENST00000382434 ENST00000382365 ENST00000446723 ENST00000315357 ENST00000400212 ENST00000306737 ENST00000382314 ENST00000382296 ENST00000449750 ENST00000415508 ENST00000440066 ENST00000382432 ENST00000400494 ENST00000382290

Gene Information

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Feb 2011]

Gene Symbol:   DAZ4
Gene Name:   deleted in azoospermia 4
Aliases:   pDP1680, pDP1681
RefSeq:   NC_000024.9 NG_004755.2 NT_011903.12
Ensembl:   ENSG00000205916
Entrez:   57135
UniGene:   Not Available
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.690000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download