PrimePCR™ Probe Assay: RNF39, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RNF39, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RNF39, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040077
Assay Design:   Intron-spanning
Chromosome Location:   6:30041233-30043119question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000425416 ENST00000449576 ENST00000256720 ENST00000396099 ENST00000396098 ENST00000400633 ENST00000293195 ENST00000544854 ENST00000420580 ENST00000581530 ENST00000583917 ENST00000442102 ENST00000582944 ENST00000413947 ENST00000455107 ENST00000456156 ENST00000442063 ENST00000441449 ENST00000450015 ENST00000327283 ENST00000591115 ENST00000353319 ENST00000591497 ENST00000309660 ENST00000394987 ENST00000383314 ENST00000592764 ENST00000593007 ENST00000262173 ENST00000451425 ENST00000432647 ENST

Gene Information

This gene lies within the major histocompatibility complex class I region on chromosome 6. Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RNF39
Gene Name:   ring finger protein 39
Aliases:   HZF, HZFW, LIRF
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204618
Entrez:   80352
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.580000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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