PrimePCR™ Probe Assay: MICA, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040062
Assay Design:   Intron-spanning
Chromosome Location:   6:31380102-31382871question
Amplicon Length:   189
Splice Variants Targeted:   ENST00000393930 ENST00000310706 ENST00000393931 ENST00000373625 ENST00000438928 ENST00000415525 ENST00000414473 ENST00000423443 ENST00000546529 ENST00000420259 ENST00000418465 ENST00000432479 ENST00000417943 ENST00000552236 ENST00000449934 ENST00000421350 ENST00000318524 ENST00000283415 ENST00000358625 ENST00000425041 ENST00000417899 ENST00000427477 ENST00000446505 ENST00000420744 ENST00000547767 ENST00000400322 ENST00000400325 ENST00000436191 ENST00000547609 ENST00000405142 ENST00000422452 ENST

Gene Information

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2010]

Gene Symbol:   MICA
Gene Name:   MHC class I polypeptide-related sequence A
Aliases:   FLJ36918, FLJ60820, MGC111087, MGC21250, MIC-A, PERB11.1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167246.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204520
Entrez:   100507436
UniGene:   Hs.130838
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.250000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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