PrimePCR™ Probe Assay: PRAMEF7, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040059
Assay Design:   Intron-spanning
Chromosome Location:   1:12978714-12979776question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000357726 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000330881 ENST00000361079 ENST00000355096 ENST00000376192 ENST00000376189 ENST00000415464 ENST00000376182 ENST00000414205 ENST00000423177 ENST00000376168 ENST00000357367 ENST00000376152 ENST00000330087

Gene Information

Description Not Available

Gene Symbol:   PRAMEF7
Gene Name:   PRAME family member 7
Aliases:   MGC178110
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000204510
Entrez:   441871
UniGene:   Hs.553832
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.490000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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