PrimePCR™ Probe Assay: C6orf27, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040039
Assay Design:   Intron-spanning
Chromosome Location:   6:31741167-31743126question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000455970 ENST00000377721 ENST00000394008 ENST00000391355 ENST00000571104 ENST00000570825 ENST00000576511 ENST00000452329 ENST00000551851 ENST00000547336 ENST00000368491 ENST00000434604 ENST00000360290 ENST00000392500 ENST00000419517 ENST00000368488 ENST00000374869 ENST00000330258 ENST00000403336 ENST00000359373 ENST00000393609 ENST00000430726 ENST00000550381 ENST00000552452 ENST00000438725 ENST00000547402 ENST00000550140 ENST00000438415 ENST00000546928 ENST00000551633 ENST00000375688 ENST

Gene Information

Description Not Available

Gene Symbol:   C6orf27
Gene Name:   chromosome 6 open reading frame 27
Aliases:   FLJ25524, G7c, NG37
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167249.1
Ensembl:   ENSG00000204396
Entrez:   80737
UniGene:   Not Available
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.300000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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