PrimePCR™ Probe Assay: SSX4B, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039999
Assay Design:   Intron-spanning
Chromosome Location:   X:48262064-48265405question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000341319 ENST00000535430 ENST00000261249 ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000306698 ENST00000419233 ENST00000263753 ENST00000383774 ENST00000382839 ENST00000324666 ENST00000374599 ENST00000374597 ENST00000252336 ENST00000357162 ENST00000358544 ENST00000395996 ENST00000378466 ENST00000376932 ENST00000319275 ENST00000376923 ENST00000311798 ENST00000403001 ENST00000347757 ENST00000376919 ENST00000376909 ENST00000298396 ENST00000376895 ENST

Gene Information

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1 SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma X breakpoint 4 SSX4 and SSX4B in tail-to-tail orientation. This gene SSX4B represents the more centromeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SSX4B
Gene Name:   synovial sarcoma, X breakpoint 4B
Aliases:   CT5.4, MGC169015, MGC169016, SSX4
RefSeq:   NC_000023.10 NG_005575.1 NT_079573.4
Ensembl:   ENSG00000198946
Entrez:   548313
UniGene:   Hs.558402
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.950000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download