PrimePCR™ Probe Assay: FAM25G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAM25G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAM25G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039989
Assay Design:   Intron-spanning
Chromosome Location:   10:48247654-48249741question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000457620 ENST00000594151 ENST00000600603 ENST00000601638 ENST00000221557 ENST00000389757 ENST00000389759 ENST00000342763 ENST00000558407 ENST00000312828 ENST00000452267 ENST00000343959 ENST00000309446 ENST00000319374 ENST00000393733 ENST00000411995 ENST00000584817 ENST00000579142

Gene Information

Description Not Available

Gene Symbol:   FAM25G
Gene Name:   family with sequence similarity 25, member G
Aliases:   KIAA0187, bA301J7.4
RefSeq:   NC_000010.10 NT_030772.10
Ensembl:   ENSG00000197910
Entrez:   100133093
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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