PrimePCR™ Probe Assay: SYNGAP1, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039986
Assay Design:   Intron-spanning
Chromosome Location:   6:33406616-33408574question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000249269 ENST00000428154 ENST00000357162 ENST00000358544 ENST00000395996 ENST00000455687 ENST00000395071 ENST00000414753 ENST00000551507 ENST00000329654 ENST00000341517 ENST00000423207 ENST00000371045 ENST00000418600 ENST00000449372 ENST00000428982 ENST00000293748 ENST00000231368

Gene Information

The protein encoded by this gene is a major component of the postsynaptic density (PSD) a group of proteins found associated with NMDA receptors at synapses. The encoded protein is phosphorylated by calmodulin-dependent protein kinase II and dephosphorylated by NMDA receptor activation. Defects in this gene are a cause of mental retardation autosomal dominant type 5 (MRD5). [provided by RefSeq Dec 2009]

Gene Symbol:   SYNGAP1
Gene Name:   synaptic Ras GTPase activating protein 1
Aliases:   DKFZp761G1421, KIAA1938, MRD5, RASA1, RASA5, SYNGAP
RefSeq:   NC_000006.11 NG_016137.1 NT_007592.15 NT_167249.1
Ensembl:   ENSG00000197283
Entrez:   8831
UniGene:   Hs.586264
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download