PrimePCR™ Probe Assay: GTF2IRD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GTF2IRD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GTF2IRD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039972
Assay Design:   Intron-spanning
Chromosome Location:   7:74247964-74251488question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000333437 ENST00000435406 ENST00000324896 ENST00000353920 ENST00000346152 ENST00000416070 ENST00000443166 ENST00000432143 ENST00000333027 ENST00000405086 ENST00000361071 ENST00000417115 ENST00000453619 ENST00000356115 ENST00000312575 ENST00000430511 ENST00000331830 ENST00000336257 ENST00000573035

Gene Information

This gene is unusual in that its coding sequence is mostly derived from Charlie8 repeat elements. However there is mRNA and EST evidence to suggest that this gene is transcribed and the encoded protein has a homolog in mouse with which it shares 78% sequence identity. The exact function of this gene product is not known. It is inferred to be a transcription factor based on the presence of GTF2I-like repeats (containing helix-loop-helix motifs) also found in other proteins such as GTF2IRD1 and GTF2I. These three genes are clustered together on chr 7q11.23. [provided by RefSeq Jul 2008]

Gene Symbol:   GTF2IRD2
Gene Name:   GTF2I repeat domain containing 2
Aliases:   FLJ21423, FLJ37938, FP630, GTF2IRD2A, MGC75203
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000196275
Entrez:   84163
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.200000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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