PrimePCR™ Probe Assay: FAM25C, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039960
Assay Design:   Intron-spanning
Chromosome Location:   10:49203487-49207816question
Amplicon Length:   181
Splice Variants Targeted:   ENST00000377069 ENST00000377071 ENST00000536437 ENST00000564962 ENST00000457620 ENST00000494664 ENST00000342763 ENST00000285039 ENST00000592779 ENST00000324581 ENST00000452267 ENST00000343959 ENST00000345136 ENST00000573514 ENST00000572449 ENST00000389347 ENST00000426333 ENST00000318967 ENST00000435409 ENST00000512605 ENST00000456435 ENST00000576422 ENST00000571768 ENST00000576030 ENST00000372706 ENST00000372705 ENST00000584817 ENST00000579142

Gene Information

Description Not Available

Gene Symbol:   FAM25C
Gene Name:   family with sequence similarity 25, member C
Aliases:   KIAA0187, bA164N7.4
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000188279
Entrez:   644054
UniGene:   Hs.645212
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.770000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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