PrimePCR™ Probe Assay: TBC1D3F, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039931
Assay Design:   Intron-spanning
Chromosome Location:   17:36288661-36290605question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000373063 ENST00000409208 ENST00000373057 ENST00000307972 ENST00000373060 ENST00000316099 ENST00000415691 ENST00000258341 ENST00000454519 ENST00000398801 ENST00000336331 ENST00000457979 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000330458 ENST00000535592 ENST00000535805 ENST00000257951 ENST00000521271 ENST00000198765 ENST00000311127 ENST00000327454 ENST00000505415 ENST00000378174 ENST

Gene Information

Description Not Available

Gene Symbol:   TBC1D3F
Gene Name:   TBC1 domain family, member 3F
Aliases:   FLJ56187, FLJ58835, TBC1D3
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000185128
Entrez:   84218
UniGene:   Hs.454716
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.250000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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