PrimePCR™ Probe Assay: FAM156B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FAM156B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FAM156B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039894
Assay Design:   Intron-spanning
Chromosome Location:   X:52929597-52936195question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000421673 ENST00000533881 ENST00000155858 ENST00000533060 ENST00000528453 ENST00000452833 ENST00000513129 ENST00000355809 ENST00000452154 ENST00000452021 ENST00000412319 ENST00000416841 ENST00000509613 ENST00000356333 ENST00000316310 ENST00000414076 ENST00000438003 ENST00000411516 ENST00000503775 ENST00000375473 ENST00000447562 ENST00000510517 ENST00000416923 ENST00000505988 ENST00000357716 ENST00000486284 ENST00000389336 ENST00000403881 ENST00000402647 ENST00000593751 ENST00000596733

Gene Information

Description Not Available

Gene Symbol:   FAM156B
Gene Name:   family with sequence similarity 156, member B
Aliases:   FLJ33130, FLJ38843, TMEM29B
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000179304
Entrez:   727866
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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