PrimePCR™ Probe Assay: LGALS7B, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039893
Assay Design:   Intron-spanning
Chromosome Location:   19:39279855-39281450question
Amplicon Length:   199
Splice Variants Targeted:   ENST00000418477 ENST00000423043 ENST00000383214 ENST00000383213 ENST00000361360 ENST00000485511 ENST00000394110 ENST00000472118 ENST00000470467 ENST00000382363 ENST00000215798 ENST00000420843 ENST00000294618 ENST00000319867 ENST00000455915 ENST00000370069 ENST00000266068 ENST00000370077 ENST00000252137 ENST00000262879 ENST00000397042 ENST00000397040 ENST00000397038 ENST00000258796 ENST00000407643 ENST00000403167 ENST00000349466 ENST00000370186 ENST00000393842 ENST00000359149 ENST00000263519 ENST

Gene Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:3963) is found adjacent to but on the opposite strand on chromosome 19. [provided by RefSeq Jul 2008]

Gene Symbol:   LGALS7B
Gene Name:   lectin, galactoside-binding, soluble, 7B
Aliases:   GAL7, Gal-7, HKL-14, LGALS7, MGC75149, MGC88745, PI7
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000178934
Entrez:   653499
UniGene:   Hs.558355
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download