PrimePCR™ Probe Assay: DMRTC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039839
Assay Design:   Intron-spanning
Chromosome Location:   X:72092295-72093428question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000454127 ENST00000334036 ENST00000373533 ENST00000373532 ENST00000333826 ENST00000373529 ENST00000373530 ENST00000290273 ENST00000597238 ENST00000596035 ENST00000594574 ENST00000601718 ENST00000596389 ENST00000595412

Gene Information

Description Not Available

Gene Symbol:   DMRTC1
Gene Name:   DMRT-like family C1
Aliases:   DMRTC1B
RefSeq:   NC_000023.10 NT_011669.17
Ensembl:   ENSG00000159123
Entrez:   63947
UniGene:   Hs.558685
Chromosome Mapping:   Xq13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.750000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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