PrimePCR™ Probe Assay: SSX2B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039838
Assay Design:   Intron-spanning
Chromosome Location:   X:52782322-52786953question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000244533 ENST00000372530 ENST00000298181 ENST00000336777 ENST00000337502 ENST00000375515 ENST00000276049 ENST00000409816 ENST00000389524 ENST00000428314 ENST00000360890 ENST00000325239 ENST00000413659 ENST00000376932 ENST00000319275 ENST00000376923 ENST00000311798 ENST00000347757 ENST00000376919 ENST00000376909 ENST00000407081 ENST00000298396 ENST00000376893 ENST00000359376 ENST00000308511 ENST00000389279 ENST00000520861 ENST00000520738 ENST00000289388 ENST00000355728 ENST00000594102 ENST

Gene Information

Description Not Available

Gene Symbol:   SSX2B
Gene Name:   synovial sarcoma, X breakpoint 2B
Aliases:   CT5.2b, SSX2
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000157950
Entrez:   727837
UniGene:   Not Available
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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