PrimePCR™ Probe Assay: RSPH10B, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039835
Assay Design:   Intron-spanning
Chromosome Location:   7:5966127-5967866question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000548523 ENST00000341698 ENST00000371650 ENST00000371652 ENST00000557021 ENST00000535557 ENST00000554546 ENST00000356296 ENST00000557597 ENST00000555238 ENST00000556772 ENST00000555394 ENST00000559312 ENST00000560335 ENST00000555738 ENST00000554521 ENST00000355058 ENST00000359560 ENST00000454166 ENST00000544991 ENST00000535282 ENST00000423334 ENST00000219313 ENST00000275162 ENST00000405415 ENST00000404406 ENST00000337579 ENST00000441023 ENST00000539903 ENST00000544099 ENST00000396908 ENST

Gene Information

Description Not Available

Gene Symbol:   RSPH10B
Gene Name:   radial spoke head 10 homolog B (Chlamydomonas)
Aliases:   MGC50833, RSPH10B2
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000155026
Entrez:   222967
UniGene:   Hs.442339
Chromosome Mapping:   7p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.970000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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