PrimePCR™ Probe Assay: TMEM236, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TMEM236, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TMEM236, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039826
Assay Design:   Intron-spanning
Chromosome Location:   10:17818056-17838018question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000569785 ENST00000254323 ENST00000590508 ENST00000440752 ENST00000358127 ENST00000325722 ENST00000381280 ENST00000220325 ENST00000343638 ENST00000302904 ENST00000373644 ENST00000337685 ENST00000377495 ENST00000338221 ENST00000480516 ENST00000457860 ENST00000377819 ENST00000546292 ENST00000538115 ENST00000542585 ENST00000546213

Gene Information

Description Not Available

Gene Symbol:   TMEM236
Gene Name:   transmembrane protein 236
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000148483
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 36.770000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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