PrimePCR™ Probe Assay: CBWD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CBWD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CBWD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039813
Assay Design:   Intron-spanning
Chromosome Location:   2:114228619-114251383question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000259199 ENST00000433343 ENST00000356521 ENST00000314367 ENST00000377391 ENST00000466582 ENST00000416428 ENST00000377457 ENST00000377449 ENST00000382399 ENST00000382405 ENST00000429800 ENST00000489273 ENST00000377392 ENST00000360171 ENST00000377344 ENST00000377339 ENST00000377342 ENST00000578449 ENST00000581344 ENST00000577357 ENST00000578761

Gene Information

Description Not Available

Gene Symbol:   CBWD2
Gene Name:   COBW domain containing 2
Aliases:   Not Available
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000136682
Entrez:   150472
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.870000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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