PrimePCR™ Probe Assay: NBPF14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NBPF14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NBPF14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039799
Assay Design:   Intron-spanning
Chromosome Location:   1:148016409-148017581question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000310771 ENST00000412624 ENST00000369365 ENST00000369372 ENST00000375552 ENST00000338347 ENST00000440491 ENST00000281815 ENST00000369339 ENST00000448873 ENST00000369338 ENST00000342960 ENST00000491285 ENST00000479926 ENST00000401010 ENST00000369323 ENST00000442909 ENST00000309471 ENST00000369226 ENST00000369228 ENST00000310701 ENST00000444640 ENST00000431121 ENST00000436356 ENST00000448574 ENST00000458135 ENST00000392972 ENST00000426874 ENST00000369219 ENST00000369202 ENST00000369188 ENST

Gene Information

Description Not Available

Gene Symbol:   NBPF14
Gene Name:   neuroblastoma breakpoint family, member 14
Aliases:   DJ328E19.C1.1, FLJ35032, FLJ60520, NBPF
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000122497
Entrez:   25832
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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