PrimePCR™ Probe Assay: UGT2B10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: UGT2B10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UGT2B10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039789
Assay Design:   Intron-spanning
Chromosome Location:   4:69693211-69696411question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000349499 ENST00000360731 ENST00000549514 ENST00000305231 ENST00000446444 ENST00000335568 ENST00000305107 ENST00000381096 ENST00000368026 ENST00000537746 ENST00000436182 ENST00000374102 ENST00000374080 ENST00000430072 ENST00000333646 ENST00000216075 ENST00000395732 ENST00000451761 ENST00000447007 ENST00000369095 ENST00000369094 ENST00000417398 ENST00000290363 ENST00000574876 ENST00000572293 ENST00000301396 ENST00000436683 ENST00000265403 ENST00000458688 ENST00000585131 ENST00000577190 ENST

Gene Information

Description Not Available

Gene Symbol:   UGT2B10
Gene Name:   UDP glucuronosyltransferase 2 family, polypeptide B10
Aliases:   MGC142209, UDPGT2B10
RefSeq:   NC_000004.11 NT_022778.16 NT_167250.1
Ensembl:   ENSG00000109181
Entrez:   7365
Chromosome Mapping:   4q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download