PrimePCR™ Probe Assay: NOMO3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NOMO3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOMO3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039787
Assay Design:   Intron-spanning
Chromosome Location:   16:16355483-16356972question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000297145 ENST00000330537 ENST00000381474 ENST00000543392 ENST00000457654 ENST00000360929 ENST00000529632 ENST00000426907 ENST00000427052 ENST00000342160 ENST00000262854 ENST00000295049 ENST00000357422 ENST00000215095 ENST00000569638 ENST00000536979 ENST00000368986 ENST00000368983 ENST00000426403 ENST00000307194 ENST00000287667 ENST00000521180 ENST00000319914 ENST00000399640 ENST00000389048 ENST00000233202 ENST00000539932 ENST00000399336 ENST00000263012 ENST00000538468 ENST00000328432 ENST

Gene Information

This gene encodes a protein originally thought to be related to the collagenase gene family. This gene is one of three highly similar genes in a duplicated region on the short arm of chromosome 16. These three genes encode closely related proteins that may have the same function. The protein encoded by one of these genes has been identified as part of a protein complex that participates in the Nodal signaling pathway during vertebrate development. Mutations in ABCC6 which is located nearby rather than mutations in this gene are associated with pseudoxanthoma elasticum. [provided by RefSeq Jul 2008]

Gene Symbol:   NOMO3
Gene Name:   NODAL modulator 3
Aliases:   Nomo
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103226
Entrez:   408050
Chromosome Mapping:   16p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.470000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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