This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MUC19, Human
PrimePCR™ Template for Probe Assay: MUC19, Human
Mucus is a viscoelastic substance that covers the epithelial surface of various tissues providing lubrication and protection from environmental insults. The gel-like properties of mucus are generally attributed to the physical properties and structural features of gel-forming mucins such as MUC19 (Chen et al. 2004 [PubMed 12882755]).[supplied by OMIM Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.